QUANTITATIVE DETERMINATION OF THE SUM OF FLAVONOIDS OF THE ABOVEGROUND PART OF SCUTELLARIA COMOSA

As a result of pharmacological studies, it was revealed that the sum flavonoids from aboveground part plant Scutellaria comosa (Lamiaceae family) has adaptogenic and antihypoxic effects. For introduction into medical practice drug based on flavonoids, studies have been conducted standardization raw materials – crested helmet. The analysis by TLC HPLC methods showed dominant flavonoid skullcap is isoscutellarein 7-O-β-D-glucopyranoside.
 A differential spectrophotometric technique developed for quantitative determination helmet in terms 7-O-β-D-glucopyranoside. optimal parameters extraction vegetable are: with 70% ethyl alcohol boiling water bath 60 minutes ratio "raw material extractant" 1 : 100, degree grinding 2 mm. It found version analysis, AlCl3 system reaches equilibrium after 40 minutes. maximum absorption spectrum observed at 346 nm can be used to analyze this group compounds.
 content varies 9.72% 10.27%. In order establish harvesting time materials, determined vegetation periods 7-O-β-D-glucopyranoside accumulation during flowering period (10.13% weight air-dry materials).